Journal: eLife

Article Title: Glycan-based shaping of the microbiota during primate evolution

doi: 10.7554/eLife.67450

Figure Lengend Snippet:

Article Snippet: Antibody , Goat Anti-Mouse Polyclonal IgM, Human ads-HRP , Southern Biotech , Cat# 1020–05, RRID: AB_2794201 , 1:4000 (ELISA).

Techniques: Probiotics, Expressing, Bacteria, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Serial Dilution, Liposomes, Control, In Vivo, Staining, Software

Journal: Experimental and Therapeutic Medicine

Article Title: Upregulated expression level of the growth factor, progranulin, is associated with the development of primary Sjögren’s syndrome

doi: 10.3892/etm.2014.1981

Figure Lengend Snippet: Quantitative polymerase chain reaction (qPCR) and immunoblot analysis. Peripheral blood mononuclear cells were separated from 26 healthy controls and 26 pSS patients prior to and following treatment with prednisone, and the total mRNA and protein were isolated. (A) Expression levels of PGRN mRNA were examined by qPCR and normalized against the expression of the GAPDH gene. (B) Protein expression levels of PGRN were examined by immunoblotting, where GAPDH was used as the loading control. Each sample was determined in triplicate and a representative blot is shown for one of the 26 patients. * P<0.05, vs. healthy controls; # P<0.05, vs. pre-treated pSS group. pSS, primary Sjögren’s syndrome; PGRN, progranulin.

Article Snippet: The primary mouse anti-human PGRN monoclonal antibody (clone 296628) was purchased from R&D Systems (Minneapolis, MN, USA), while the primary mouse anti-GAPDH monoclonal and secondary horseradish peroxidase-conjugated goat anti-mouse antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Techniques: Real-time Polymerase Chain Reaction, Western Blot, Isolation, Expressing, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Upregulated expression level of the growth factor, progranulin, is associated with the development of primary Sjögren’s syndrome

doi: 10.3892/etm.2014.1981

Figure Lengend Snippet: Comparison of serum levels of PGRN and IL-6 by ELISA.

Article Snippet: The primary mouse anti-human PGRN monoclonal antibody (clone 296628) was purchased from R&D Systems (Minneapolis, MN, USA), while the primary mouse anti-GAPDH monoclonal and secondary horseradish peroxidase-conjugated goat anti-mouse antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Techniques: Comparison, Enzyme-linked Immunosorbent Assay, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Upregulated expression level of the growth factor, progranulin, is associated with the development of primary Sjögren’s syndrome

doi: 10.3892/etm.2014.1981

Figure Lengend Snippet: Spearman’s rank correlation analyses of PGRN and IL-6 levels in pSS patients (A) prior to and (B) following treatment with prednisone. P<0.05 indicates a statistically significant difference. PGRN, progranulin; IL-6, interleukin-6; pSS, primary Sjögren’s syndrome.

Article Snippet: The primary mouse anti-human PGRN monoclonal antibody (clone 296628) was purchased from R&D Systems (Minneapolis, MN, USA), while the primary mouse anti-GAPDH monoclonal and secondary horseradish peroxidase-conjugated goat anti-mouse antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Techniques:

Journal: Stem Cell Research & Therapy

Article Title: Pentosan polysulfate binds to STRO-1 + mesenchymal progenitor cells, is internalized, and modifies gene expression: a novel approach of pre-programing stem cells for therapeutic application requiring their chondrogenesis

doi: 10.1186/s13287-017-0723-y

Figure Lengend Snippet: Primary and secondary antibodies used for MPC ± PPS cytometric analysis

Article Snippet: IgM FITC , Goat anti-mouse IgM FITC , Southern Biotech 1020-02.

Techniques: Purification, Control

Journal: Stem Cell Research & Therapy

Article Title: Pentosan polysulfate binds to STRO-1 + mesenchymal progenitor cells, is internalized, and modifies gene expression: a novel approach of pre-programing stem cells for therapeutic application requiring their chondrogenesis

doi: 10.1186/s13287-017-0723-y

Figure Lengend Snippet: Concentration-dependent uptake of PPS-FITC by MPCs (2.5 × 10 5 cells/well) determined using the fluorometric assay. Highest uptake was observed with PPS-FITC concentrations of 5.0 μg/ml which were significantly different to 1.0 and 2.5 μg/ml. a p < 0.004; b p < 0.012. FITC fluorescein isothiocyanate, PPS pentosan polysulfate

Article Snippet: IgM FITC , Goat anti-mouse IgM FITC , Southern Biotech 1020-02.

Techniques: Concentration Assay

Journal: Stem Cell Research & Therapy

Article Title: Pentosan polysulfate binds to STRO-1 + mesenchymal progenitor cells, is internalized, and modifies gene expression: a novel approach of pre-programing stem cells for therapeutic application requiring their chondrogenesis

doi: 10.1186/s13287-017-0723-y

Figure Lengend Snippet: Fluorescence microscopy images of MPCs (6000/well) cultured with 2.5 μg/ml PPS-FITC for 24 h, fixed in Histochoice MB/ethanol and stained with propidium iodide. a A 2-s exposure image of the MPCs outlined by the low-level background autofluorescence and the higher emission arising from the PPS-FITC located within the cells ( arrows ). b The same field as a , but with 60-s exposure with excitation at 535 nm confirming the presence of PPS-FITC within the nucleus of the MPCs ( arrows ). FITC fluorescein isothiocyanate, PPS pentosan polysulfate

Article Snippet: IgM FITC , Goat anti-mouse IgM FITC , Southern Biotech 1020-02.

Techniques: Fluorescence, Microscopy, Cell Culture, Staining

Journal: iScience

Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers

doi: 10.1016/j.isci.2023.106310

Figure Lengend Snippet:

Article Snippet: Alexa Fluor 555 Goat anti-IgM (Polyclonal) , SouthernBiotech , Cat# 1020–32; RRID: AB_2794223.

Techniques: Purification, Recombinant, Virus, Saline, Plasmid Preparation, Microscopy, Reverse Transcription, Software